CROT (N-term) Rabbit Polyclonal Antibody

CAT#: AP51088PU-N

CROT (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of CROT Antibody (N-term) in K562 cell line and mouse liver tissue lysates (35ug/lane). CROT (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human brain tissue reacted with CROT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  CROT Antibody (N-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blot: 1/50 - 1/100.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Flow cytometry: 1/10 - 1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 57~86 amino acids from the N-terminal region of human CROT
• Specificity: This antibody reacts to human and mouse CROT.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 70178 Da
• Gene Name: carnitine O-octanoyltransferase
• Database Link:
  Entrez Gene 74114 MouseEntrez Gene 54677 Human
  UniProt ID Q9UKG9
• Background: CROT encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.
• Synonyms: COT

Reference Data

• Protein Families: Druggable Genome