AMY2B (N-term) Rabbit Polyclonal Antibody

CAT#: AP50167PU-N

AMY2B (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

AMY2B Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the AMY2B antibody detected the AMY2B protein (arrow).

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  AMY2B antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AMY2B antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of AMY2B Antibody (N-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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  AMY2B Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blotting: 1/100 - 1/500.
  Immunohistochemistry: 1/50 - 1/100.
  Immunofluorescence: 1/10 - 1/50.
  Flow Cytometry: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 77-105 amino acids from the N-terminal region of human AMY2B
• Specificity: This antibody reacts to Alpha-amylase 2B.
• Formulation: PBS containing 0.09% (W/V) sodium azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: amylase, alpha 2B (pancreatic)
• Database Link:
  Entrez Gene 280 Human
  UniProt ID P19961
• Background: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas.
• Synonyms: Carcinoid alpha-amylase
• Note: Molecular Weight: 57710 Da

Reference Data

• Protein Families: Secreted Protein
• Protein Pathways: Metabolic pathways, Starch and sucrose metabolism