Synaptopodin (SYNPO) (N-term) Guinea Pig Polyclonal Antibody

CAT#: AP33487SU-N

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Synaptopodin (SYNPO) (N-term) guinea pig polyclonal antibody, Serum



USD 570.00

2 Weeks*

Size
    • 100 ul

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Specifications

Product Data
Applications IF, IHC, WB
Recommended Dilution Immunoblotting (Western): 1/500-1/1000.
Immunofluorescence.
Immunohistochemistry on Frozen Sections:
1/50-1/100.
Immunohistochemistry on Paraffin Sections: 1/50-1/100 (after microwave treatment). 
Incubation Time:1 h at RT for immunohistochemistry.
Reactivities Human, Mouse
Host Guinea Pig
Clonality Polyclonal
Immunogen Synthetic peptides (Mouse N-terminus), coupled to KLH
Specificity The antibody reacts specifically with Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin belongs to actin-binding proteins, it has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the cor-responding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (attributed to post-translational modifications). In Western blot analysis the antibody also reacts with a 44 kD degradation fragment of synaptopodin. 
The antibody recognizes differentiated podocytes (glomerular visceral epi-thelial cells) in vivo and in vitro (weaker additional reaction with arterial endo-thelial cells), co-localization with alpha-actinin.
Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of murine brain. Differentia-tion-dependent expression in cultured hippocampal neurons.
Formulation State: Serum
State: Liquid Stabilized Antiserum
Preservative: 0.09% Sodium Azide
Stabilizer: 0.5 % BSA
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Stability Shelf life: one year from despatch.
Synonyms KIAA1029
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.