NPTX1 Goat Polyclonal Antibody

CAT#: AP32971PU-N

NPTX1 goat polyclonal antibody, Aff - Purified

Product Images

(1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Peptide ELISA: 1/128000 (Detection Limit).
  Western blot: 1-3 µg/ml. Approx 50kDa band observed in Human Brain (Amygdala and Frontal Cortex) lysates (calculated MW of 47.1kDa according to NP_002513.2).
  Additional bands of unknown identity was also consistently observed at 38 and 14kDa. These bands were successfully blocked by incubation with the immunizing peptide.
  Immunohistochemistry on Paraffin Sections: 5µg/ml. Paraffin embedded Human Brain (Cerebellum).
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-ERVKIETALTSLHQR, from the internal region of the protein sequence according to NP_002513.2.
• Specificity: This antibody recognizes Neuronal pentraxin-1.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: neuronal pentraxin 1
• Database Link:
  Entrez Gene 4884 Human
  UniProt ID Q15818
• Synonyms: Neuronal pentraxin-1, Nptx1, NP1