HIC1 (193-204) Goat Polyclonal Antibody

CAT#: AP32955PU-N

HIC1 (193-204) goat polyclonal antibody, Aff - Purified

Product Images

HEK293 overexpressing Human HIC1 and probed with HIC1 Antibody -N (Left) and (Right) at 0.5 ug/ml with the mock transfection in second lane. Data obtained from Dr. D. Leprince, CNRS UMR 8161, Institut de Biologie de LILLE, France.

• 

  (1 ug/ml) staining of A549 (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: 1/128000 (Detection Limit).
  Western blot: 0.5-1 μg/ml. In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kDa is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kDa according to NP_006488.2.
• Reactivities: Canine, Human, Mouse, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_006488.2; NP_001091672.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_006488.2; NP_001091672.1).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: hypermethylated in cancer 1
• Database Link:
  Entrez Gene 3090 Human
  UniProt ID Q14526
• Synonyms: HIC-1