PKC beta 1 (PRKCB) Goat Polyclonal Antibody

CAT#: AP32819PU-N

PKC beta 1 (PRKCB) goat polyclonal antibody, Aff - Purified

Product Images

PRKCB Antibody (0.1 ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  PRKCB Antibody (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/128000 (Detection Limit).
  Western blot: 0.1-0.3 μg/ml. Approx 80kDa band observed in Human Placenta lysates (calculated MW of 76.9kDa according to NP_997700.1).
  Immunohistochemistry on Paraffin Sections: 4-6 µg/ml. In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes.
• Reactivities: Canine, Human, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region (near C-terminus) of the protein sequence according to NP_997700.1.
• Specificity: This antibody is expected to recognize isoform 1 (NP_997700.1) only.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: protein kinase C beta
• Database Link:
  Entrez Gene 5579 Human
  UniProt ID P05771
• Synonyms: PKC-beta, PKC beta, PKC-B, PKCB, PRKCB1, Protein kinase C beta type