PRAK (MAPKAPK5) (192-206) Goat Polyclonal Antibody

SKU AP32809PU-N

PRAK (MAPKAPK5) (192-206) goat polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, IHC, IP, WB
• Recommended Dilution: Peptide ELISA: 1/64000 (Detection Limit).; Western blot: 0.5-1 μg/ml. In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP; only the expected band was observed in the MK5 lane after AP32809PU-N labeling. ; Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. ; Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 µg AP32808PU-N or AP32809PU-N. ; Western blots of the Immunoprecipitation were labelled with Mouse anti-MK5 (SC) or with Rabbit anti-ERK3 serum (CST). An approx 55kDa of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression constructas described in PMID: 22508986.; Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.; Immunohistochemistry on Paraffin Sections: 4-8 µg/ml. In paraffin embedded Human Kidney shows nuclear staining in the DCT.
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region (192-206) of the protein sequence according to NP_003659.2; NP_620777.1.
• Specificity: Recognizes MK5 / MAPKAPK5 (192-206).
• Buffer: Tris saline, pH~7.3, 0; State: Aff - Purified; State: Liquid purified Ig fraction; Stabilizer: 0.5% BSA; Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Shipping: Blue Ice
• Gene Name: mitogen-activated protein kinase-activated protein kinase 5
• Database Link:
  Q8IW41
• Synonyms: MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK5, PRAK

Reference Data