LC3 pSer12 Rabbit Polyclonal Antibody

SKU AP32191PU-N

LC3 pSer12 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: WB
• Recommended Dilution: Western Blot: 1/1000.; Dot Blot: 1/500.
• Reactivity: Human
• Antibody Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic phosphopeptide between 1-30 amino acids surrounding Ser12 of Human LC3 (APG8a).
• Specificity: Recognizes Phospho LC3C- Serine12
• Buffer: PBS; State: Aff - Purified; State: Liquid purified Ig fraction; Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: 14272 Da
• Gene Name: microtubule associated protein 1 light chain 3 alpha
• Database Link:
  NP_115903.1
  Q9H492
• Background: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
• Synonyms: MAP1A / 1B light chain 3 A, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1 light chain 3-like protein 1, MAP1LC3A, LC3A

Reference Data