AP2 alpha (AP2A1) (706-727) Goat Polyclonal Antibody

SKU AP32069PU-N

AP2 alpha (AP2A1) (706-727) goat polyclonal antibody, Aff - Purified

Product Data

• Application: IHC, PEP-ELISA, WB
• Recommended Dilution:

  Peptide ELISA: 1/128000 (Detection Limit).; Western blot: 0.01-0.03 μg/ml. Approx 110kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kDa according to NP_055018.2). The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates.; Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane.;

• Reactivity: Bovine, Human, Mouse, Porcine, Rat
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_055018.2.
• Specificity:
  This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.
• Buffer: Tris saline, pH~7.3; State: Aff - Purified; State: Liquid purified Ig fraction; Stabilizer: 0.5% BSA; Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Shipping: Blue Ice
• Gene Name: adaptor related protein complex 2 alpha 1 subunit
• Database Link:
  O95782
• Synonyms: CLAPA1, Alpha adaptin A, Alpha-1 adaptin, AP2 complex subunit alpha-1

Reference Data