PATJ (N-term) Goat Polyclonal Antibody

SKU AP31982PU-N

PATJ (N-term) goat polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, IHC
• Recommended Dilution:

  Peptide ELISA: 1/32000 (Detection limit).; Western blot: Preliminary experiments in Human Brain and Kidney lysates consistently showed a strong band at 100kDa followed by bands at ~55kDa, 45kDa and 40kDa at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the bands we observe given the predicted size of approx. 196Kda according to NP_795352.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Is this protein particularly susceptible to proteolysis? ; Immunohistochemistry on Paraffin Sections: 3-6 µg/ml. In paraffin embedded Human Testis shows strong staining of spermatids.

• Reactivity: Bovine, Canine, Human, Porcine
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide with sequence from the N-Terminus of Human INADL (NP_795352.2).
• Specificity: This antibody detects INADL/PATJ at N-term.
• Buffer: Tris saline, pH~7.3; State: Aff - Purified; State: Liquid purified Ig fraction; Stabilizer: 0.5% BSA; Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Shipping: Blue Ice
• Gene Name: PATJ, crumbs cell polarity complex component
• Database Link:
  Q8NI35
• Background: This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors. Alternative splicing results in four transcript variants encoding different isoforms. Additional transcript variants have been described but their full-length sequences have not been determined.
• Synonyms: PATJ

Reference Data