VPS11 Goat Polyclonal Antibody

SKU AP31975PU-N

VPS11 goat polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, IHC
• Recommended Dilution:
  Peptide ELISA: 1/32000 (Detection limit). ; Western blot: Preliminary experiments gave an approx 75kDa band in Human duodenum, colon and placenta lysates after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 108kDa according to NP_068375.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. ; Immunohistochemistry on Paraffin Sections: 3-6 μg/ml. In paraffin embedded Human Kidney shows brushborder staning in PCT cells and Human Pancreas shows cytoplasm staining of beta cells in islets of Langerhans.
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_068375.3.
• Specificity: This antibody recognizes RNF108 / VPS11.
• Buffer: Tris saline, pH~7.3; State: Aff - Purified; State: Liquid purified Ig fraction; Stabilizer: 0.5% BSA; Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Shipping: Blue Ice
• Gene Name: VPS11, CORVET/HOPS core subunit
• Database Link:
  Q9H270
• Background: Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.
• Synonyms: PP3476

Reference Data