E2 230K (UBE2O) (C-term) Goat Polyclonal Antibody

CAT#: AP31922PU-N

E2 230K (UBE2O) (C-term) goat polyclonal antibody, Aff - Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human Heart stained with UBE2O antibody at 4 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: ELISA: 1/32000 (Detection Limit).
  Western blot: Preliminary experiments in Human Colon lysates and in lysastes of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
  Immunohistochemistry on Paraffin Sections: 4-6 μg/ml.
  In paraffin embedded Human Heart shows textured staining in some myocardial fibres.
• Reactivities: Bovine, Canine, Human, Mouse, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: UBE2O antibody was raised against synthetic peptide (ALLEAGMPECTEDK) from C-terminus of Human UBE2O (NP_071349.3)
• Specificity: Recognizes UBE2O (C-term).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ubiquitin conjugating enzyme E2 O
• Database Link:
  Entrez Gene 63893 Human
  UniProt ID Q9C0C9
• Synonyms: Ubiquitin-conjugating enzyme E2 O, Ubiquitin-protein ligase O, Ubiquitin carrier protein O, E2-230K, KIAA1734