E2 230K (UBE2O) (C-term) Goat Polyclonal Antibody
Product Data | |
Application | ELISA, IHC |
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Recommended Dilution | ELISA: 1/32000 (Detection Limit). Western blot: Preliminary experiments in Human Colon lysates and in lysastes of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Immunohistochemistry on Paraffin Sections: 4-6 μg/ml. In paraffin embedded Human Heart shows textured staining in some myocardial fibres. |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Antibody Host | Goat |
Clonality | Polyclonal |
Immunogen | UBE2O antibody was raised against synthetic peptide (ALLEAGMPECTEDK) from C-terminus of Human UBE2O (NP_071349.3) |
Specificity | Recognizes UBE2O (C-term). |
Buffer | Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide |
Concentration | lot specific |
Purification | Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide |
Conjugation | Unconjugated |
Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Shipping | Blue Ice |
Gene Name | ubiquitin conjugating enzyme E2 O |
Database Link | |
Synonyms | Ubiquitin-conjugating enzyme E2 O, Ubiquitin-protein ligase O, Ubiquitin carrier protein O, E2-230K, KIAA1734 |
Reference Data |
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