DsbA-L Rabbit Polyclonal Antibody

SKU AP31632SU-N

DsbA-L rabbit polyclonal antibody, Serum

Product Data

• Application: IHC, WB
• Recommended Dilution: Western Blot: 1/1000.; Immunohistochemistry: 1/200.
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Full length recombinant Mouse DsbA-L protein.
• Specificity: Specific for the ~25 kDa DsbA-L protein in Western blots of 3T3-L1 adipocytes and Mouse white and brown adipose tissue lysate samples. It is suggested that you add 1% BSA to the antibody dilution buffer.
• Buffer: State: Serum; State: Liquid Serum in 40% Glycerol.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted (in aliquots) at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Background: Disulfide-bond-A oxidoreductase-like protein (DsbA-L, previously named as GST Kappa) is an adiponectin-interacting protein. DsbA-L is highly expressed in adipose tissue, and its expression level is negatively correlated with obesity in mice and humans. DsbA-L expression in 3T3-L1 adipocytes is stimulated by the insulin sensitizer; rosiglitazone and inhibited by the inflammatory cytokine TNFalpha. Polymorphism of DsbA-L gene has recently been implicated in insulin secretion and body fat distribution (Gao F et al., 2009). Overexpression of DsbA-L promotes adiponectin multimerization while suppressing DsbA-L expression by RNAi markedly and selectively reduces adiponectin levels and secretion in 3T3-L1 adipocytes. Recent studies identify DsbA-L as a key regulator for adiponectin biosynthesis (Liu et al., 2008).
• Synonyms: Disulfide-bond-A oxidoreductase-like protein

Reference Data