p114RhoGEF (ARHGEF18) (C-term) Goat Polyclonal Antibody

CAT#: AP31062PU-N

p114RhoGEF (ARHGEF18) (C-term) goat polyclonal antibody, Aff - Purified

Product Images

AP31062PU-N (0.5ug/ml) staining of lysates of cell lines Daudi (A) and Caco-2 (B) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: 1/128000 (Detection Limit).
  Western Blot: This antibody has been successfully used for Caco-2, HCE and MDCK cells as described by Terry SJ et al in Reference 1.
• Reactivities: Canine, Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_056133.2; NP_001124427.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_056133.2; NP_001124427.1).
• Formulation: Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
  State: Aff - Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Rho/Rac guanine nucleotide exchange factor 18
• Database Link:
  Entrez Gene 23370 Human
  UniProt ID Q6ZSZ5
• Synonyms: KIAA0521, p114-Rho-GEF