TRIP (TRAIP) (C-term) Goat Polyclonal Antibody

CAT#: AP31057PU-N

TRIP (TRAIP) (C-term) goat polyclonal antibody, Aff - Purified

Product Images

TRAIP antibody staining of Human Testis lyssate at 2 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  TRAIP antibody staining of paraffin embedded Human Spleen at 4 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/1000 (Detection Limit).
  Western Blot: 2.0 µg/ml. Approx.50kDa band observed in Human Testis lysates.
  An additonal band of unkown identity was also concistently observeved at 38kDa. This band was successfully blocked by incubation with the immunizing peptide.
  Immunohistochemistry on Paraffin Sections: 4-6 µg/ml. In paraffin embedded Human Spleen shows perinucxlear and textured cystoplasmic staining in selective areas.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the C-Terminus of the protein sequence according to NP_005870.2.
• Specificity: This antibody detects TRIP / TRAIP
• Formulation: Tris saline, pH 7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction.
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 53.3 kDa (NP_005870.2).
• Gene Name: TRAF interacting protein
• Database Link:
  Entrez Gene 10293 Human
  UniProt ID Q9BWF2
• Background: TRAIP is a protein that contains an N-terminal RING finger motif and a putative coiled-coil domain. A similar murine protein interacts with TNFR-associated factor 1 (TRAF1), TNFR-associated factor 2 (TRAF2), and cylindromatosis. The interaction with TRAF2 inhibits TRAF2-mediated nuclear factor kappa-B, subunit 1 activation that is required for cell activation and protection against apoptosis.
• Synonyms: TRIP, TRAF-interacting protein

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency