PDCD10 Rabbit Polyclonal Antibody
PDCD10 rabbit polyclonal antibody, Aff - Purified
|Recommended Dilution||Western Blot: 1-5 μg/ml.
Immunofluorescence: 2-10 µg/ml.
|Immunogen||Highly pure (> 95%) recombinant Human CCM3, amino acids Met1-Ala212 derived from E.coli (Cat.-No AR26004PU-N)|
|Specificity||This antibody will detect recombinant Human CCM-3 in Western Blot and native CCM-3 in Immunohistochemistry.|
|Formulation||5mM PBS, pH 7.2 without preservatives or stabilizers
State: Aff - Purified
State: Lyophilized purified IgG fraction
|Reconstitution Method||Restore with sterile water to a concentration of 1.0 mg/ml.|
|Purification||Affinity Chromatography with Immobilized Protein A|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Gene Name||Homo sapiens programmed cell death 10 (PDCD10), transcript variant 1|
|Background||Cerebral cavernous malformations (CCMs) are sporadically acquired or inherited vascular lesions of the central nervous system consisting of clusters of dilated thin-walled blood vessels that predispose individuals to seizures and stroke. Mutations in CCM1, CCM2, or CCM3 lead to cerebral cavernous malformations, one of the most common hereditary vascular diseases of the brain. Endothelial cells within these lesions are the main disease compartments. Here, we show that adenoviral CCM3 expression inhibits endothelial cell migration, proliferation, and tube formation while down regulation of endogenous CCM3 results in increased formation of tube-like structures. Adenoviral CCM3 expression does not induce apoptosis under normal endothelial cell culture conditions but protects endothelial cells from staurosporine-induced cell death. Tyrosine kinase activity profiling suggests that CCM3 supports PDPK-1/Akt-mediated endothelial cell quiescence and survival (Schleider et al, Neurogenetics 12, 2011).
The CCM-3 is fused to a N-terminal His-tag (6x His).
|Protein Families||Druggable Genome|
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