GLUD1 (+Glutamate dehydrogenase 2) Goat Polyclonal Antibody
GLUD1 (+Glutamate dehydrogenase 2) goat polyclonal antibody, Aff - Purified
|Applications||ELISA, IF, IHC, WB|
|Recommended Dilution||Peptide ELISA: 1/32000 (Detection Limit).
Western blot: 0.3-1 µg/ml. Approx 55kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1, of 61.3kDa according to Mouse NP_032159.1 and of 61.4kDa according to Rat NP_036702.1).
Immunofluorescence: Customer found particulate cytoplasm staining in HeLa.
Immunohistochemistry on Paraffin Sections: 3-5 µg/ml. In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons.
|Reactivities||Bovine, Canine, Human, Mouse, Rat|
|Immunogen||Peptide with sequence from the internal region of the protein sequence according to NP_005262.1; NP_036216.2.|
|Specificity||This antibody recognizes Glutamate Dehydrogenase.
This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).
|Formulation||Tris saline, pH~7.3
State: Aff - Purified
State: Liquid purified Ig fraction
Stabilizer: 0.5% BSA
Preservative: 0.02% Sodium Azide
|Purification||Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide|
|Storage||Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Gene Name||Homo sapiens glutamate dehydrogenase 1 (GLUD1), transcript variant 1|
|Synonyms||GLUD1, GLUD, GDH1|
|Protein Families||Druggable Genome|
|Protein Pathways||Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism|