GLUD1 (+Glutamate dehydrogenase 2) Goat Polyclonal Antibody

CAT#: AP23724PU-N

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GLUD1 (+Glutamate dehydrogenase 2) goat polyclonal antibody, Aff - Purified



USD 429.00


Availability*
5 Days

Size
    • 100 ug


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Specifications

Product Data
Applications ELISA, IF, IHC, WB
Recommended Dilution Peptide ELISA: 1/32000 (Detection Limit). 
Western blot:  0.3-1 µg/ml. Approx 55kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1, of 61.3kDa according to Mouse NP_032159.1 and of 61.4kDa according to Rat NP_036702.1).
Immunofluorescence: Customer found particulate cytoplasm staining in HeLa. 
Immunohistochemistry on Paraffin Sections:  3-5 µg/ml. In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons.
Reactivity Bovine, Canine, Human, Mouse, Rat
Host Goat
Clonality Polyclonal
Immunogen Peptide with sequence from the internal region of the protein sequence according to NP_005262.1; NP_036216.2.
Specificity This antibody recognizes Glutamate Dehydrogenase.
This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).
Formulation Tris saline, pH~7.3
State: Aff - Purified
State: Liquid purified Ig fraction
Stabilizer: 0.5% BSA
Preservative: 0.02% Sodium Azide
Concentration lot specific
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens glutamate dehydrogenase 1 (GLUD1), transcript variant 1
Synonyms GLUD1, GLUD, GDH1
Reference Data
Protein Families Druggable Genome
Protein Pathways Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism
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