Smoothened (SMO) (N-term) Rabbit Polyclonal Antibody

CAT#: AP23648PU-N

Smoothened (SMO) (N-term) rabbit polyclonal antibody

Product Images

Western blot analysis of extracts from HuvEc cells, using SMO Antibody. The lane on the right is treated with the synthesized peptide.

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  Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Immunohistochemistry on Paraffin Sections: 1/100.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: SMO antibody was raised against synthetic peptide - KLH conjugated
• Specificity: This antibody reacts to the N-term of Smoothened Homolog (SMOH).
• Formulation: PBS (without Mg2+, Ca2+), pH 7.4 containing 150 mM sodium chloride, 0.02% sodium azide as preservative and 50% glycerol as stabilizer
  State: Liquid purfied Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: smoothened, frizzled class receptor
• Database Link:
  Entrez Gene 25273 RatEntrez Gene 319757 MouseEntrez Gene 6608 Human
  UniProt ID Q99835
• Background: Smoothened, a Frizzled Receptor, is homologous to the Drosophila segment polarity Smo gene. It associates with the Patched protein to mediate the cellular response to the Hedgehog secreted protein signal, which controls patterning and growth during vertebrate development. Smoothened is activated in a number of human tumors and can function as an oncogene in basal-cell carcinomas.
• Synonyms: SMOH, Smoothened homolog

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transmembrane
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer