ACTN2 (215-500) Rabbit Polyclonal Antibody

CAT#: AP23620PU-N

ACTN2 (215-500) rabbit polyclonal antibody, Aff - Purified

Product Images

Sample (30 ug of whole cell lysate). A: A431. B: H1299. 7.5% SDS PAGE. ACTN2 antibody diluted at 1/1000.

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  Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using actinin, alpha 2 antibody at 1/200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunocytochemistry.
  Immunofluorescence: 1/100 - 1/200.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot: 1/500 - 1/3000.
• Reactivities: Bovine, Human, Mouse, Rat, Zebrafish
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 215 and 500 of actinin, alpha 2
• Specificity: This antibody reacts to alpha Actinin 2 at aa 215-500.
• Formulation: 0.1 M Tris-glycine, pH 7.0 containing 10% Glycerol and 0.01% Thimerosal
  State: Aff - Purified
  State: Liquid purfied Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: actinin alpha 2
• Database Link:
  Entrez Gene 11472 MouseEntrez Gene 291245 RatEntrez Gene 88 Human
  UniProt ID P35609
• Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane.
• Synonyms: Alpha actinin skeletal muscle isoform 2

Reference Data

• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction