NSE (ENO2) (C-term) Rabbit Polyclonal Antibody
Product Data | |
Application | IF, IHC, IP, WB |
---|---|
Recommended Dilution | Western blot: At 1-2μg/ml with the appropriate system to detect NSE in cells and tissues. Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect NSE in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immuncytochemistry. Immunprecipitation. |
Reactivity | Human, Mouse, Rat |
Antibody Host | Rabbit |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | Peptide mapping at the C-terminus of NSE of human origin |
Specificity | This antibody detects Neuron specific enolase at C-term. |
Buffer | 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction |
Reconstitution Method | 0.2ml of distilled water will yield a concentration of 500μg/ml. |
Purification | Immunogen affinity purified |
Conjugation | Unconjugated |
Storage | Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Shipping | Ambient |
Gene Name | enolase 2 |
Database Link | |
Background | NSE (neuron specific enolase), also known as Enolase 2(ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13. |
Synonyms | NSE, ENO2, Enolase 2,Neural enolase, Gamma-enolase |
Reference Data | |
Protein Pathways | Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation |
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