OB Cadherin (CDH11) (497-509) Goat Polyclonal Antibody

CAT#: AP21504PU-N

OB Cadherin (CDH11) (497-509) goat polyclonal antibody, Aff - Purified

Product Images

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CDH11 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: Detection limit: 1/8000.
  Western blot: 0.1-0.5 µg/ml. In transfected HEK293 transiently expressing full-length Human CDH11 (myc and DYKDDDDK tagged), a band of approx. 130kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.
• Reactivities: Bovine, Canine, Human, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_001788.2.
• Specificity: Recognizes CDH11 (aa 497-509).
• Formulation: Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cadherin 11
• Database Link:
  Entrez Gene 1009 Human
  UniProt ID P55287
• Synonyms: Osteoblast cadherin, OSF-4, CDH11, OB Cadherin