Prostatic Acid Phosphatase (ACPP) Rabbit Polyclonal Antibody
| Product Data | |
| Application | ELISA, ID, IF, IP, R, WB |
|---|---|
| Recommended Dilution | This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques). Working dilutions in non-precipitating antibody-binding techniques: 1/100-1/1,000. |
| Reactivity | Human |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Acid Phosphatase isolated and purified from Human seminal plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
| Specificity | Acid Phosphatase from Human seminal plasma. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined |
| Buffer | PBS, pH 7.2 without preservatives and foreign proteins. Label: Biotin State: Lyophilized hyperimmune IgG fraction. Molar radio: Biotin/IgG ~ 6.0 |
| Reconstitution Method | Restore by adding 1.0 ml of sterile distilled water |
| Concentration | lot specific |
| Purification | Ammonium Sulphate Precipitation and Ion Exchange Chromatography. |
| Conjugation | Biotin |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Gene Name | acid phosphatase, prostate |
| Database Link | |
| Background | Prostatic acid phosphatase is one of the two antigenic markers of prostatic carcinoma, the other being prostate specific antigen. It belongs to the kallikrein family of serine proteases and is suggested to act as a hydrolase to split phospharyl choline in semen and as a transferase. |
| Synonyms | Prostate acid phosphatase, PAP, ACP3, PSAP |
| Reference Data | |
| Protein Families | Druggable Genome, Phosphatase, Transmembrane |
| Protein Pathways | Riboflavin metabolism |
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