ETFB (152-165) Goat Polyclonal Antibody
Product Data | |
Application | ELISA, IHC, WB |
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Recommended Dilution | Peptide ELISA: 1/8000 (Detetion Limit). Western blot: 1-3 µg/ml. This antibody detects a band of ~27kDa in Human, Mouse and Rat Liver lysates. Immunohistochemistry on Paraffin Sections: 3-5 µg/ml. In paraffin embedded Human Liver shows mitochondrial staining in hepatocytes. |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Antibody Host | Goat |
Clonality | Polyclonal |
Immunogen | Peptide with sequence from the internal region (aa 152-165) of the protein sequence according to NP_001976.1 and NP_001014763.1. |
Specificity | This antibody is expected to recognize both reported isoforms (NP_001976.1 and NP_001014763.1). |
Buffer | Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer. State: Aff - Purified State: Liquid purified Ig fraction. |
Concentration | lot specific |
Purification | Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide. |
Conjugation | Unconjugated |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Shipping | Blue Ice |
Gene Name | electron transfer flavoprotein beta subunit |
Database Link | |
Background | ETFB is a is an electron-transfer-flavoprotein, (beta polypeptide), and a subcomponent of Electron transfer flavoprotein (ETF). ETF exists in the mitochondrial matrix as a heterodimer of 30-kD alpha subunits (ETFA) and 28-kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer. This electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases and transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). |
Synonyms | Beta-ETF |
Note | Calculated Molecular Weight: 27.8kDa (NP_001976.1). |
Reference Data | |
Protein Families | Druggable Genome |
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