MT ND5 (ND5) Rabbit Polyclonal Antibody

CAT#: AP21196PU-N

MT ND5 (ND5) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of ND5 antibody in extracts from HT-29 cells.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 321-370 of Human ND5.
• Specificity: This antibody detects endogenous levels of ND5 protein.
  (region surrounding Met359)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen (>95% pure by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 67 kDa
• Gene Name: mitochondrially encoded NADH dehydrogenase 5
• Database Link:
  Entrez Gene 4540 Human
  UniProt ID P03915
• Background: A small number of complex I subunits are the products of mitochondrial genes (subunits 1-7), while the remainder are nuclear encoded and imported from the cytoplasm. NADH dehydrogenase subunit 5 (ND5) is most likely a component of the hydrophobic protein fragment of Complex I. Mutations in the gene encodiing for ND5 are implicated in Mitochondrial neurogastrointestinal encephalomyopathy, an autosomal recessive multisystem disorder that results in dramatically elevated levels of circulating thymidine and deoxyuridine.
• Synonyms: NADH-ubiquinone oxidoreductase chain 5, MT-ND5, MTND5, NADH5