ZIP Kinase (DAPK3) Rabbit Polyclonal Antibody

CAT#: AP21094PU-N

ZIP Kinase (DAPK3) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of ZIP-kinase antibody (Cat.-No.: AP21094PU-N) in extracts from HuvEc cells.

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  Immunohistochemistry (IHC) analyzes of ZIP-kinase antibody (Cat.-No.: AP21094PU-N) in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of ZIPkinase protein.
  (region surrounding Lys261)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 52 kDa
• Gene Name: death associated protein kinase 3
• Database Link:
  Entrez Gene 13144 MouseEntrez Gene 64391 RatEntrez Gene 1613 Human
  UniProt ID O43293
• Background: Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certainserine/threonine protein kinases, such as ASK1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N terminal kinase domain and a C terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREBfamily. ZIP kinase has high sequence homology to DAP kinase (death associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.
• Synonyms: DAP kinase 3, ZIP-kinase, DLK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Bladder cancer, Pathways in cancer