DGKE Rabbit Polyclonal Antibody

SKU AP20421PU-N

DGKE rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.; Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of DGK-ε protein.; (region surrounding Ser194)
• Buffer: Phosphate buffered saline (PBS), pH 7.2; State: Aff - Purified; State: Liquid purified Ig fraction; Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: ~ 60 kDa
• Gene Name: diacylglycerol kinase epsilon
• Database Link:
  P52429
• Background: Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-α (80 kDa), -β (90 kDa), and -γ (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-δ (130 kDa) and DGK-?(130 kDa) contain N-terminal pleckstrin homology (PH) domains and are classified as type II. Human DGK-ε (64 kDa) contains no identifiable regulatory domains and is classified as a type III DGK. Human DGK-ζ (104 kDa) and -iota (130 kDa) possess C-terminal ankyrin repeats and are classified as type IV DGKs. Human DGK-θ (110 kDa) contains 3 cysteine-rich domains and a PH domain and is classified as a type V DGK.
• Synonyms: Diacylglycerol kinase epsilon, DGKE, DAGK5, Diglyceride kinase epsilon, DGK-epsilon

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system