Separase (ESPL1) Rabbit Polyclonal Antibody

CAT#: AP20283PU-N

Separase (ESPL1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Separase antibody (9AP20283PU-N) in extracts from 293 cells.

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  Immunohistochemistry analyzes of Separase antibody (AP20283PU-N) in paraffin-embedded human colon carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunoflourescence: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Separase protein.
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 230 kDa
• Gene Name: extra spindle pole bodies like 1, separase
• Database Link:
  Entrez Gene 105988 MouseEntrez Gene 9700 Human
  UniProt ID Q14674
• Background: Separase is a cysteine protease that triggers anaphase in all eukaryotes by participating in separation of sister chromatids during mitosis. Once activated, separase hydrolyzes the SCC1 subunit of cohesin, the chromosomal protein complex responsible for sister chromatid cohesion. Separase and cohesin are highly conserved from yeasts to humans. When the cell is not dividing, separase is prevented from cleaving cohesin through its association with another protein, securin. When anaphase is signaled, the securin is ubiquitinated and hydrolyzed by APC/cyclosome, releasing the active separase. Separase is transiently activated between the two meioses and may also be involved in homolog separation.
• Synonyms: Separase, ESP1, KIAA0165

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Cell cycle, Oocyte meiosis