PI4KB (Center) Rabbit Polyclonal Antibody

CAT#: AP17647PU-N

PI4KB (Center) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of PIK4CB Antibody (Center) in Hela cell line lysates (35ug/lane). PIK4CB (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PIK4CB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  PIK4CB Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: Western blotting: 1/1000.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Bovine, Human, Mouse, Rat, Zebrafish
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 519-548 amino acids from the Center region of Human PIK4CB.
• Specificity: This antibody recognizes PIK4CB.
• Formulation: PBS with 0.09% (W/V) Sodium Azide
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: phosphatidylinositol 4-kinase beta
• Database Link:
  Entrez Gene 81747 RatEntrez Gene 107650 MouseEntrez Gene 5298 Human
  UniProt ID Q9UBF8
• Background: PIK4CB phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). It may regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation.
• Synonyms: PIK4CB, PI4K-beta, PI4Kbeta, PtdIns 4-kinase beta, NPIK, PI4K92

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system