LRRC32 Rabbit Polyclonal Antibody

CAT#: AP17415PU-N

LRRC32 rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of GARP antibody (Center) ) in CEM cell line (Left) and Mouse cerebellum tissue (Right) lysates (35 ug/lane). GARP (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded (from Left to Right) human hepatocarcinoma, lung carcinoma and cerebellum reacted with GARP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 234~260 amino acids from the Center region of Human GARP.
• Specificity: This antibody detects GARP at the center region.
  Cellular Location: Membrane; Single-pass type I membrane protein.
  Tissue Location: Preferentially expressed in regulatory T-cells (T(regs)).
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20 °C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 71979 Da
• Gene Name: Homo sapiens leucine rich repeat containing 32 (LRRC32), transcript variant 2
• Database Link:
  Entrez Gene 434215 MouseEntrez Gene 2615 Human
  UniProt ID Q14392
• Background: GARP, also known as leucine rich repeat containing 32, is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region. GARP was identified based on the analysis of gene expression profiling of Treg and Th cells following TCR stimulation.
• Synonyms: Garpin, D11S833E

Reference Data

• Protein Families: Transmembrane