ALAD (C-term) Rabbit Polyclonal Antibody

CAT#: AP17099PU-N

ALAD (C-term) rabbit polyclonal antibody

Product Images

Western blot analysis of ALAD Antibody (C-term) in Jurkat cell line lysates (35 ug/lane). ALAD (arrow) was detected using the purified Pab.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1:50 - 1:100.
  ELISA: 1:1,000.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide selected from the C-terminal region of human ALAD
• Specificity: This antibody detects ALAD at C-term.
• Formulation: PBS with 0.121% (W/V) sodium azide
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: aminolevulinate dehydratase
• Database Link:
  Entrez Gene 210 Human
  UniProt ID P13716
• Background: The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria.
• Synonyms: Delta-aminolevulinic acid dehydratase, ALADH, Porphobilinogen synthase
• Note: Molecular weight: 36295 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Porphyrin and chlorophyll metabolism