NIR2 (PITPNM1) (C-term) Goat Polyclonal Antibody

SKU AP16227PU-N

NIR2 (PITPNM1) (C-term) goat polyclonal antibody, Aff - Purified

Product Data

• Application: IF, WB
• Recommended Dilution: Peptide ELISA: Limit dilution 1:8000.; Western blot: Customer's experiments gave a band at approx 160kDa in lysates of a Mouse Macrophage cell line after 2 µg/ml antibody staining (calculated MW of 135kDa according to NP_004901.1). ; Immunocytochemistry: The customer found staining of vesicular cytoplasm in the same cell line.
• Reactivity: Bovine, Canine, Human, Mouse
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide from the C-Terminus of the protein sequence according to NP_004901.2; NP_001124320.1.
• Specificity: This antibody detects NIR2 at C-term and is expected to recognise both reported isoforms.
• Buffer: Tris saline, pH~7.3; State: Aff - Purified; State: Liquid purified Ig fraction; Stabilizer: 0.5% BSA; Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Shipping: Blue Ice
• Gene Name: phosphatidylinositol transfer protein membrane associated 1
• Database Link:
  O00562
• Background: NIR2 belongs to a family of proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein. Nir2, a human homolog of Drosophila melanogaster retinal degeneration B protein, is essential for cytokinesis.; A specific Thr residue in the Nir2 PI-transfer domain provides a regulatory site for targeting to lipid droplets. This may affect intracellular lipid trafficking and distribution & explain the dominant effect of the RdgB-T59E mutant on retinal degeneration.
• Synonyms: PITPNM1, DRES9, NIR2, PITPNM

Reference Data