PTPN5 (N-term) Rabbit Polyclonal Antibody

SKU AP15239PU-N

PTPN5 (N-term) rabbit polyclonal antibody, Purified

Product Data

• Application: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.; Western blotting: 1/100 - 1/500.; Immunohistochemistry: 1/10 - 1/50.
• Reactivity: Human
• Antibody Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human STEP.
• Specificity: This antibody reacts to STEP.
• Buffer: PBS with 0.09% (W/V) sodium azide; State: Purified; State: Liquid purified Ig
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: protein tyrosine phosphatase, non-receptor type 5
• Database Link:
  P54829
• Background: STEP (striatum-enriched phosphatase) is a neural-specific protein-tyrosine phosphatase, first isolated from the rat brain. The 537-amino acid predicted human protein as isolated from cDNA sequences is between 85 and 90% identical to the mouse and rat sequences. In rat neuronal cell cultures, glutamate-mediated activation of N-methyl-D-aspartate (NMDA) receptors leads to the rapid but transient phosphorylation of extracellular signal-related kinase-2 (ERK2). NMDA-mediated influx of calcium, activates the calcium-dependent phosphatase calcineurin and the resulting dephosphorylation and activation of STEP. STEP then inactivatea ERK2 through tyrosine dephosphorylation and blocks translocation of the kinase to the nucleus. STEP plays a significant role in regulating the ERK activation and downstream signaling in neurons.
• Synonyms: FLJ14427, STEP61, STEP46, STEP38, STEP20

Reference Data