PAK5 Rabbit Polyclonal Antibody

CAT#: AP14809PU-N

PAK5 rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-PAK5 Pab in lysates from transiently transfected COS7 cells. Lane 1: negative control, Lane 2: PAK1-expressing cells, Lane 3: PAK2-expressing cells, Lane 4: PAK4-expressing cells, Lane 5: PAK5-expressing cells, and Lane 6: PAK6-expressing cells. PAK5 (arrow) was detected using purified Pab.

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  Formalin-fixed and paraffin-embedded human brain tissue reacted with PAK5 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100 - 1/500.
  Immunoprecipitation: 1/100.
  Immunohistochemistry: 1/10 - 1/50.
  Flow cytometry.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from aa 183~198 of human PAK5.
• Specificity: This antibody reacts to PAK5.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: p21 (RAC1) activated kinase 7
• Database Link:
  Entrez Gene 57144 Human
  UniProt ID Q9P286
• Background: PAK5 is a member of the PAK family of Ser/Thr protein kinases. PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. This kinase is associated with microtubule networks and induces microtubule stabilization. The subcellular localization of this kinase is tightly regulated during cell cycle progression.
• Synonyms: p21-activated kinase 7, PAK-7, p21-activated kinase 5, PAK-5, KIAA1264