ketohexokinase (KHK) (N-term) Rabbit Polyclonal Antibody

CAT#: AP13633PU-N

ketohexokinase (KHK) (N-term) rabbit polyclonal antibody, Purified

Product Images

The anti-KHK Pab is used in Western blot to detect KHK in mouse liver tissue lysate (Lane 1) and 293 cell lysate (Lane 2).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100 - 1/500.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human KHK.
• Specificity: This antibody reacts to Ketohexokinase (KHK).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Purified polyclonal antibody supplied in . This antibody is purified through a
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ketohexokinase
• Database Link:
  Entrez Gene 3795 Human
  UniProt ID P50053
• Background: Ketohexokinase (KHK), or fructokinase, catalyzes conversion of fructose to fructose-1-phosphate. Splice variant 1 is the highly active form found in liver, renal cortex, and small intestine, while splice variant 2 is the lower activity form found in most other tissues. KHK, like glucokinase (GCK) and glucokinase regulator (GCKR), is present in both liver and pancreatic islets. The inhibition of GCK by GCKR is blocked by binding of fructose-1-phosphate to GCKR. The chromosomal proximity of the metabolically connected GCKR and KHK genes has a genetic linkage in type 2 diabetes. Fructosuria, or hepatic fructokinase deficiency, is a benign, asymptomatic defect of intermediary metabolism associated with heterozygosity for G50R and A43T mutations in KHK.
• Synonyms: KHK, Ketohexokinase, EC=2.7.1.3, Hepatic fructokinase