Semaphorin 5A (SEMA5A) (N-term) Rabbit Polyclonal Antibody

CAT#: AP12324PU-N

Semaphorin 5A (SEMA5A) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-Sema5a (N-term) Pab in A2058 cell line lysates (35ug/lane). Sema5a (arrow) was detected using the purified Pab (1:60 dilution).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Peptide ELISA: 1/1,000.
  Western blot: 1/1000.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide selected from the N-terminal region of Mouse Sema5a.
• Specificity: This antibody detects Sema5a (N-term).
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Protein A Chromatography followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: semaphorin 5A
• Database Link:
  Entrez Gene 9037 Human
  UniProt ID Q13591
• Background: Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.
  Members of the semaphorin protein family, such as SEMA5A, are involved in axonal guidance during neural development.
• Synonyms: Semaphorin-5A, Semaphorin-F, Sema F, SEMAF
• Note: Molecular weight: 120321 Da

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Axon guidance