Aconitase 2 (ACO2) Rabbit Polyclonal Antibody

CAT#: AP11745PU-N

Aconitase 2 (ACO2) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-ACO2 in mouse heart (left) and 293 (right) tissue lysates (35 ug/lane). ACO2 (arrow) was detected using the purified Pab (1:60 dilution).

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  Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent, containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail; PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2 (Center). Solution: 1:1000 diluted in 5% NFM TBS-T 0.05 for overnight (15 hrs) at 4°C. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.

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  Formalin-fixed and paraffin-embedded human Heart tissue reacted with ACO2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 438-467 amino acids from the central region of Human Aconitase.
• Specificity: This antibody detects ACO2 at Center.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 85425 Da
• Gene Name: aconitase 2
• Database Link:
  Entrez Gene 50 Human
  UniProt ID Q99798
• Background: ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.
• Synonyms: Aconitase 2