SIGLEC8 (C-term) Rabbit Polyclonal Antibody

SKU AP11632PU-N

SIGLEC8 (C-term) rabbit polyclonal antibody, Purified

Product Data

• Application: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.; Western blot: 1/1000.; Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 373-406 amino acids from the C-terminal region of Human SIGLEC8.
• Specificity: This antibody detects SIGLEC8 at C-term.
• Buffer: PBS with 0.09% (W/V) Sodium Azide; State: Purified; State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: sialic acid binding Ig like lectin 8
• Database Link:
  Q9NYZ4
• Background: SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-linked sialic acid. and also binds to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.
• Synonyms: Siglec-8, CDw329, SAF2
• Note: Molecular weight: 54042 Da

Reference Data