GCAP1 (GUCA1A) (C-term) Rabbit Polyclonal Antibody

SKU AP11600PU-N

GCAP1 (GUCA1A) (C-term) rabbit polyclonal antibody, Purified

Product Data

• Application: IHC, WB
• Recommended Dilution: ELISA 1:1,000.; Western blot 1:100 - 1:500.; Immunohistochemistry 1:50 - 1:100.
• Reactivity: Human
• Antibody Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human GCAP1.
• Specificity: This antibody detects GCAP1 at C-term.
• Buffer: PBS with 0.09% (W/V) sodium azide; State: Purified; State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: guanylate cyclase activator 1A
• Database Link:
  P43080
• Background: Guanylate cyclase-activating protein is a l Ca(2+)-binding protein that upregulates synthesis of cGMP in photoreceptors. The known mammalian GCAPs are more than 90% similar, consisting of 201 to 205 amino acids, and containing 3 identically conserved Ca(2+)-binding sites. The GUCA1A gene, also termed GCAP1, is transcribed into a single 1.7-kb mRNA species detectable only in the retina. In a 4-generation British family with typical clinical features of autosomal dominant cone dystrophy a tyr99-to-cys mutation) in the GUCA1A gene has been identified. Another family with a pro50-to-leu mutation in GUCA1A demonstrated phenotypic variability ranging from mild photophobia to rod-cone dystrophy. The mutant protein could activate guanylate cyclase 1 (GUCY2D) and displayed similar calcium sensitivity to wildtype protein. However, there was a marked increase in the susceptibility to protease degradation and a reduction in the thermal stability of the pro50-to-leu mutation, which may depress cellular concentration and thereby contribute to retinal cell mortality.
• Synonyms: Guanylyl cyclase-activating protein 1, GUCA1, C6orf131, Guanylate cyclase activator 1A
• Note: Molecular weight: 22774 Da

Reference Data