Frequenin (NCS1) Rabbit Polyclonal Antibody

CAT#: AP11588PU-N

Frequenin (NCS1) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of NCS1 Antibody (Center) in MCF-7 cell line lysates (35 ug/lane). NCS1 (arrow) was detected using the purified Pab.

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  Formalin-Fixed and Paraffin-Embedded Human breast carcinoma reacted with NCS1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Flow Cytometric analysis of ZR-75-1 cells using NCS1 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blot: 1/1,000.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Bovine, Chicken, Human, Mouse, Rat, Xenopus
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 118-144 amino acids from the Central region of Human NCS1.
• Specificity: This antibody detects NCS1 at center.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 21747 Da
• Gene Name: neuronal calcium sensor 1
• Database Link:
  Entrez Gene 23413 Human
  UniProt ID P62166
• Background: NCS1 is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. NCS1 regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. This protein is thought to be associated with secretory granules and may be involved in the regulation of neurosecretion.
• Synonyms: NCS-1, FREQ, FLUP