KREMEN1 (N-term) Rabbit Polyclonal Antibody

CAT#: AP11560PU-N

KREMEN1 (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of Kremen (arrow) using Kremen Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Kremen gene (Lane 2) (Origene Technologies).

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  Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA 1:1,000.
  Western blot 1:100 - 1:500.
  Immunohistochemistry 1:50 - 1:100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Kremen.
• Specificity: This antibody detects Kremen at N-term.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: kringle containing transmembrane protein 1
• Database Link:
  Entrez Gene 83999 Human
  UniProt ID Q96MU8
• Background: Kremen is a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein is a component of a membrane complex that modulates canonical WNT signaling through lipoprotein receptor-related protein 6 (LRP6). It contains extracellular kringle, WSC, and CUB domains.
• Synonyms: KREMEN1, KREMEN, KRM1, Dickkopf receptor
• Note: Molecular weight: 63,201 Da (Isoform 3)