Proteasome 20S alpha 5 (PSMA5) (Center) Rabbit Polyclonal Antibody

CAT#: AP11429PU-N

Proteasome 20S alpha 5 (PSMA5) (Center) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of PSMA5 (arrow) using rabbit polyclonal PSMA5 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2) (Origene Technologies).

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  Western blot analysis of PSMA5 Antibody (Center) in HL60 cell line lysates (35 ug/lane). PSMA5 (arrow) was detected using the purified polyclonal antibody.

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  Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PSMA5 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA 1:1,000.
  Western blot 1:50 - 1:100.
  Immunohistochemistry 1:10 - 1:50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human PSMA5.
• Specificity: This antiboidy detects PSMA5 at center.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: proteasome subunit alpha 5
• Database Link:
  Entrez Gene 5686 Human
  UniProt ID P28066
• Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PMA5 is a member of the peptidase T1A family, that is a 20S core alpha subunit.
• Synonyms: Proteasome subunit alpha type-5, Proteasome 20S alpha 5, Macropain zeta chain, Proteasome zeta chain
• Note: Molecular weight: 26411 Da