CDCA8 Rabbit Polyclonal Antibody

CAT#: AP11424PU-N

CDCA8 rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of CDCA8 Antibody (Center) in HL60 cell line lysates (35 ug/lane). CDCA8 (arrow) was detected using the purified polyclonal antibody.

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  Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CDCA8 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA 1:1,000.
  Western blotting 1:100 - 1:500.
  Immunohistochemistry 1:10 - 1:50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human CDCA8.
• Specificity: This antibody detects CDCA8 at center.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cell division cycle associated 8
• Database Link:
  Entrez Gene 55143 Human
  UniProt ID Q53HL2
• Background: CDCA8 is a component of the chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The CPC complex, a key regulator of mitosis, has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, CDCA8 may be required to direct the CPC to centromeric DNA.
• Synonyms: Dasra-B, hDasra-B, PESCRG3
• Note: Molecular weight: 31323 Da