RIZ1 (PRDM2) Rabbit Polyclonal Antibody

CAT#: AP11193PU-N

RIZ1 (PRDM2) rabbit polyclonal antibody, Purified

Product Images

Immunohistochemical analysis of RIZ1 in adenovirus-transduced tumor cells. Established HCT116 xenograft tumors at day 2 after infection of AdRIZ1, Adnull, or buffer alone were processed for RIZ1 in situ detection (TUNEL) analysis.

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  Rat B50 brain tumor cell extracts were immunoprecipitated with RIZ antibody or preimmune.

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western Blot: 1/100-1/500.
  Immunoprecipitation: 1 µg Pab/100 µg cell lysate.
  Immunohistochemistry: 1/100-1/500.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Purified recombinant GST fusion protein encoding aa 245-573 of Rat Riz1.
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: PR domain 2
• Database Link:
  Entrez Gene 7799 Human
  UniProt ID Q13029
• Background: PRDM2 is a 250-kD nuclear protein containing 8 zinc finger motifs. It contains an RB-binding motif that is related to that of the adenovirus E1A oncoprotein; RIZ also shares an antigenic epitope with the C terminus of E1A. It is expressed in human retinoblastoma cells and at low levels in all other human cell lines examined. Using fluorescence in situ hybridization, the PRDM2 gene has been mapped to 1p36. PRDM2 may be a candidate target of 1p36 alterations that commonly occur in neuroendocrine, breast, liver, colon, skin, and lymphoid tumors.
• Synonyms: PR domain zinc finger protein 2, KMT8, RIZ1, RIZ2, MTB-ZF, HUMHOXY1