NOS1 (1422-1433) Rabbit Polyclonal Antibody

SKU AP08236PU-N

NOS1 (1422-1433) rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IF, IHC, IP, WB
• Recommended Dilution: Immunocytochemistry.; Immunohistochemistry on Paraffin Sections: 5 µg/ml.; Immunoprecipitation.; Western Blot.
• Reactivity: Bat, Canine, Equine, Human, Monkey, Mouse, Rabbit, Rat
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Human nNOS amino acids 1422-1433
• Specificity: This antibody is specific to nNOS amino acids 1422-1433.
• Buffer: TBS, pH 7.4, containing 50% glycerol and 0.02% sodium azide; State: Aff - Purified; State: Liquid purified Ig fraction
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: nitric oxide synthase 1
• Database Link:
  P29475
• Background: Three isoforms of nitric oxide synthase (NOS) have been identified. All are homodimers with subunits of 130-160 kDa. All have binding sites for NADPH, FAD, and FMN near the carboxyl terminus (the reductase domain), and binding sites for tetrahydrobiopterin (BH4) and heme near the amino terminus (the oxygenase domain). The reductase and oxygenase domains are linked by a calmodulin (CaM) binding site. Occupation of this site facilitates electron transfer from the cofactors in the reductase domain to heme during nitric oxide production. NOS catalyzes the conversion of arginine to citrulline and nitric oxide (NO). Neuronal nitric oxide synthase (nNOS, bNOS, cNOS, Type I) is associated with the post-synaptic density protein (PSD-95) in the neuronal membrane. In response to increased intracellular Ca2+, nNOS interacts with CaM. The Ca2+ CaM complex, in combination with BH4, binds to nNOS and induces its translocation from the plasma membrane to the cytoplasm. The dephosphorylation of nNOS by calcineurin initiates the production NO. NO activates guanylyl cyclase (GC) and activates the various cGMP regulated signaling pathways. nNOS is in activated by phosphorylation by protein kinase A (PKA) or protein kinase C (PKC).
• Synonyms: bNOS, nNOS, NOS type I, Neuronal NOS, Constitutive NOS, NC-NOS

Reference Data