Collagen VI (COL6A1) Rabbit Polyclonal Antibody
| Product Data | |
| Application | ELISA, IF, IHC, IP, WB |
|---|---|
| Recommended Dilution | ELISA. Immunofluorescence. Immunohistochemistry on Paraffin Sections: 2.5 µg/ml. Immunoprecipitation. Western Blot. |
| Reactivity | Bovine, Human |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Collagen Type VI from Human and Bovine placenta |
| Specificity | This antibody detects Collagen, Type VI from Human and Bovine Placenta. Other species not tested. |
| Buffer | 0.02 M Potassium Phosphate, pH 7.2, 0.15 M Sodium Chloride with 0.01% (w/v) Sodium Azide as preservative State: Aff - Purified State: Liquid purified IgG fraction |
| Concentration | lot specific |
| Purification | Immunoaffinity Chromatography |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | collagen type VI alpha 1 |
| Database Link | |
| Background | Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used. |
| Synonyms | COL6A1 |
| Reference Data | |
Write Your Own Review
| Product Manuals |
| FAQs |
| SDS |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.