POLK Goat Polyclonal Antibody

SKU AP07619PU-N

POLK goat polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/2000 - 1/10000.; Immunofluorescence.; Immunohistochemistry on Paraffin Sections: 5 µg/ml.; Western Blot: 1/500 - 1/3000.
• Reactivity: Human, Mouse
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to Amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.; State: Aff - Purified; State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted (in aliquots) at -20°C.; Dilute only prior to immediate use.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: polymerase (DNA) kappa
• Database Link:
  Q9UBT6
• Background: POLK (also known as DNA polymerase kappa, DINB protein and DINP) is involved in DNA repair. POLK plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK lacks 3'-5' proofreading exonuclease activity. POLK is a nuclear protein that binds to REV1L and PCNA. POLK is detected throughout the nucleus and at replication foci. Multiple alternative splice variants have been detected for this protein. POLK is found at low levels in testis, spleen, prostate and ovary and at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
• Synonyms: POLK, DINB1, DINB protein, DINP

Reference Data