MNAT1 Rabbit Polyclonal Antibody

SKU AP06703PU-N

MNAT1 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.; Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human MNAT1.
• Specificity: This antibody detects endogenous levels of Mat1 protein.; (region surrounding Glu123)
• Buffer: Phosphate buffered saline (PBS), pH 7.2.; State: Aff - Purified; State: Liquid purified Ig fraction; Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: ~ 36 kDa
• Gene Name: MNAT1, CDK activating kinase assembly factor
• Database Link:
  P51948
• Background: Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit, Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires phosphorylation at Thr 160. The enzyme responsible for phosphorylation of Thr 160 in Cdk2 and also Thr 161 in Cdc2 p34, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit, a regulatory subunit and a subunit of unknown function. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. This previously undescribed protein, now termed Mat1 p36, has been cloned as a protein that associates with the cyclin H/Cdk7 nuclear complex at all stages of the cell cycle. Cyclin H/Cdk7/Mat1 p36 complexes display kinase activity towards Cdk activation domains, and the carboxy terminus of RNA polymerase II. Mat1 p36 appears to constitute the first example of an assembly factor, essential for the formation of an active Cdk/cyclin complex.
• Synonyms: RING finger protein MAT1, RING finger protein 66, Menage a trois, CAP35, MAT1

Reference Data