CLOCK Rabbit Polyclonal Antibody

CAT#: AP06636PU-N

CLOCK rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Clock antibody in extracts from HUVEC cells.

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  Immunohistochemistry analyzes of Clock antibody in paraffin-embedded human skeletal muscle tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 241-290 of Human Clock.
• Specificity: This antibody detects endogenous levels of Clock protein.
  (region surrounding Glu270)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~100 kDa
• Gene Name: clock circadian regulator
• Database Link:
  Entrez Gene 9575 Human
  UniProt ID O15516
• Background: Clock, a member of the basic-helix-loop-helix-PAS (bHLH-PAS) family of transcription factors, has also been identified as having circadian function. Mutations within the Clock gene have been shown to increase the length of the endogenous period and to cause a loss of rhythmicity of circadian oscillations. Clock contains a DNA-binding domain, a protein dimerization domain and a glutamine-rich C-terminal region, which indicates transactivation capabilities. It has been speculated that Clock may regulate circadian rhythmicity in combination with other proteins such as Per.
• Synonyms: KIAA0334, BHLHE8