PKC delta (PRKCD) Rabbit Polyclonal Antibody

CAT#: AP06564PU-N

PKC delta (PRKCD) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of PKC ?´ antibody in extracts from MCF7 cells.

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  Immunohistochemistry (IHC) analyzes of PKC ?´ antibody in paraffin-embedded human brast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 470-520 of Human PKC δ.
• Specificity: This antibody detects endogenous levels of PKC delta protein.
  (region surrouding Gly499)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 80 kDa
• Gene Name: protein kinase C delta
• Database Link:
  Entrez Gene 5580 Human
  UniProt ID Q05655
• Background: Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.
• Synonyms: PKC-delta, PKC delta, PKCD, Protein kinase C delta type