NCF4 Rabbit Polyclonal Antibody
| Product Data | |
| Application | IHC, WB |
|---|---|
| Recommended Dilution | Western blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200. |
| Reactivity | Human, Mouse |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide, corresponding to amino acids 115-170 of Human p40-phox. |
| Specificity | This antibody detects endogenous levels of p40 phox protein. (region surrounding Arg149) |
| Buffer | Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% sodium azide |
| Concentration | 1.0 mg/ml |
| Purification | Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Predicted Protein Size | ~ 39 kDa |
| Gene Name | neutrophil cytosolic factor 4 |
| Database Link | |
| Background | Nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase is a multimeric enzyme system that mediates electron transport from NADPH in the cytoplasm to molecular oxygen in the phagosome, thereby generating reactive oxidant intermediates. Upon neutrophil stimulation, NADPH-oxidase and other cytosolic elements localize to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. There are a number of cytosolic proteins that are involved in NADPH-oxidase activation/deactivation,including p47-phox, p67-phox, p40-phox and the small GTP-binding protein, Rac. Activation of NADPH oxidase is accompanied by the phosphorylation of cytosolic components p40-phox, p47-phox and p67-phox. The PKC consensus phosphorylation sites Thr 154 and Ser 315 in p40-phox are phosphorylated during activation of NADPH oxidase. p40-phox can promote oxidase activation by increasing the affinity of p47-phox for NADPH-oxidase. However, p40-phox appears to downregulate oxidase function as well, by competing with an SH3 domain interaction between other essential oxidase components. |
| Synonyms | Neutrophil cytosol factor 4, SH3PXD4, p40-phox |
| Reference Data | |
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